This comes in two major forms. The person either does get frozen but becomes Un-Paused before they should, or it's a No-Sell on the entire effect. This is not always a simple matter of just being immune though, sometimes it's part of Applied Phlebotinum, sheer willpower, or some sort of dumb luck of having something else interfere with the effect. It also happens a lot when characters with Super Speed are in play; from their perspective, the world is always moving slowly, so all the time-stopper does is even the playing field. If a time stop is visually represented by the user's surroundings going monochrome or negative, expect this character to return to their normal colour. May come with an Imminent Danger Clue in the form of a "frozen" person's fingers or eyes moving slightly.
A limitation of stable transfection is time in which a practical amount of protein can be produced and utilized in experiments. To try and overcome this, the current protocol describes a culturing step that can achieve substantial amounts of protein within 3-6 weeks of transfection. The batch overgrow cultures (large scale production) provide the opportunity to produce substantial amounts of antibody for the setup of in vitro characterization experiments in the lead up to selection of a clonal cell line and lead candidates. This has clear advantages over transient transfection, which can require higher amounts of DNA and reagents. In addition, the protein yield is not reproducible from batch to batch since expression is only temporary and determined by transfection efficiency.
Time Stopper 3.5 Setup Freel
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